A Synthetic Hydrogel, VitroGel ® ORGANOID-3, Improves Immune Cell-Epithelial Interactions in a Tissue Chip Co-Culture Model of Human Gastric Organoids and Dendritic Cells

A Synthetic Hydrogel, VitroGel ® ORGANOID-3, Improves Immune Cell-Epithelial Interactions in a Tissue Chip Co-Culture Model of Human Gastric Organoids and Dendritic Cells
Immunosurveillance of the gastrointestinal epithelium by mononuclear phagocytes (MNPs) is crucial for sustaining intestine well being. Nonetheless, learning the advanced interaction between the human gastrointestinal epithelium and MNPs reminiscent of dendritic cells (DCs) is troublesome, since conventional cell tradition methods lack complexity, and animal fashions could not adequately characterize human tissues.
Microphysiological methods, or tissue chips, are a pretty various for these investigations, as a result of they mannequin practical options of particular tissues or organs utilizing microscale tradition platforms that recreate physiological tissue microenvironments.
Nonetheless, profitable integration of a number of of tissue sorts on a tissue chip platform to breed physiological cell-cell interactions stays a problem. We beforehand developed a tissue chip system, the intestine organoid circulation chip (GOFlowChip), for long run tradition of 3-D pluripotent stem cell-derived human intestinal organoids.
Right here, we optimized the GOFlowChip platform to construct a posh microphysiological immune-cell-epithelial cell co-culture mannequin in an effort to examine DC-epithelial interactions in human abdomen. We first examined totally different tubing supplies and chip configurations to optimize DC loading onto the GOFlowChip and demonstrated that DC tradition on the GOFlowChip for as much as 20 h didn’t impression DC activation standing or viability.
Nonetheless, Transwell chemotaxis assays and dwell confocal imaging revealed that Matrigel, the extracellular matrix (ECM) materials generally used for organoid tradition, prevented DC migration in direction of the organoids and the institution of direct MNP-epithelial contacts.
Subsequently, we subsequent evaluated DC chemotaxis by way of various ECM supplies together with Matrigel-collagen mixtures and artificial hydrogels. A polysaccharide-based artificial hydrogel, VitroGel®-ORGANOID-3 (V-ORG-3), enabled considerably elevated DC chemotaxis by way of the matrix, supported organoid survival and development, and didn’t considerably alter DC activation or viability.
On the GOFlowChip, DCs that had been flowed into the chip migrated quickly by way of the V-ORG matrix and reached organoids embedded deep throughout the chip, with elevated interactions between DCs and gastric organoids.
The profitable integration of DCs and V-ORG-Three embedded gastric organoids into the GOFlowChip platform now permits real-time imaging of MNP-epithelial interactions and different investigations of the advanced interaction between gastrointestinal MNPs and epithelial cells of their response to pathogens, candidate medication and mucosal vaccines.
Dysfunction of epithelial permeability barrier induced by HMGB1 in 2.5D cultures of human epithelial cells
Airway and intestinal epithelial permeability boundaries are essential in epithelial homeostasis. Excessive mobility group field 1 (HMGB1), elevated by varied stimuli, is concerned within the induction of airway irritation, in addition to the pathogenesis of inflammatory bowel illness.
HMGB1 enhances epithelial hyperpermeability. Two-and-a-half dimensional (2.5D) tradition assays are experimentally handy and induce cells to type a extra physiological tissue structure than 2D tradition assays for molecular switch mechanism evaluation.
In 2.5D tradition, remedy with HMGB1 induced permeability of FITC-dextran into the lumen shaped by human lung, nasal and intestinal epithelial cells. The tricellular tight junction molecule angulin-1/LSR is liable for the epithelial permeability barrier at tricellular contacts and contributes to numerous human airway and intestinal inflammatory illnesses.
On this assessment, we point out the mechanisms together with angulin-1/LSR and a number of signaling in dysfunction of the epithelial permeability barrier induced by HMGB1 in 2.5D tradition of human airway and intestinal epithelial cells.
Human platelet lysate effectivity, stability, and optimum heparin focus required in tradition of mammalian cells.
Fetal bovine serum (FBS) has been used to help the expansion and proliferation of mammalian cells for many years. Owing to a number of threat components related to FBS, a number of trials have been performed to guage substitutes to FBS with the identical effectivity and the decrease threat points.
On this examine, human platelet lysate (HPL) derived from activated human platelets was evaluated as a substitute for FBS as a result of related threat components. To judge the effectivity of the preparation course of, platelet rely was carried out earlier than and after activation.
The concentrations of a number of development components and proteins had been measured to analyze HPL effectivity. HPL stability was studied at common intervals, and optimum heparin focus required to stop gel formation in varied media was decided. The organic exercise of HPL and FBS was in contrast by evaluating the expansion efficiency of Vero and Hep-2 cell strains.
Results of platelet rely assay revealed the effectivity of HPL preparation course of. Progress issue concentrations in HPL had been considerably increased than these in FBS, whereas the protein content material of HPL was decrease than that of FBS.
Stability examine knowledge confirmed that the ready HPL was secure for as much as 15 months at -20℃. Supreme heparin focus for use in several media was depending on calcium focus. Outcomes of cell viability assay confirmed that HPL was superior to FBS in supporting the expansion and proliferation of Vero and Hep-2 cells.The HPL ready by the mechanical activation of platelets could function an environment friendly various to FBS in cell tradition course of.
A Synthetic Hydrogel, VitroGel ® ORGANOID-3, Improves Immune Cell-Epithelial Interactions in a Tissue Chip Co-Culture Model of Human Gastric Organoids and Dendritic Cells

Tradition substrates product of elastomeric micro-tripod arrays for long-term growth of human pluripotent stem cells.

Culturing and growth of human pluripotent stem cells (hPSCs) depend upon the chemical and bodily properties of the substrates. Gel-coated substrates offering low stiffness are generally used for long-term pluripotency upkeep of hPSCs.
We beforehand reported that gelatin nanofibrous substrates additionally permit long-term culturing of hPSCs, suggesting the significance of three-dimensional (3D) group of extracellular matrix proteins. To additional consider the importance of 3D options and materials stiffness, we fabricated elastomeric micro-tripod arrays (MTAs) and maintained hPSC propagation over 10 passages with out observing the pluripotency loss or the event of chromosomal abnormality.
We additionally discovered that the hPSC colonies on MTAs had been extra rounded than these on gel-coated flat substrates. Theoretical analyses prompt that the efficient stiffness of elastomeric MTAs is akin to that of gel-coated substrates and that the impact of structural anisotropy is negligible. Cells on MTAs profit from enhanced diffusion beneath the hPSC colonies in addition to enzyme-free detachment from the substrate throughout passage.

Chemically fastened autologous feeder cell-derived area of interest for human induced pluripotent stem cell tradition.

Typical tradition of human induced pluripotent (hiPS) cells requires feeder cell preparation that’s time consuming and labour intensive. Alternatively, feeder-free tradition of hiPS cells requires excessive cell seeding densities, specialised tradition medium, and development dietary supplements, which elevate the associated fee.
Moreover, recombinant methods require a very long time for colony formation. Right here, we employed chemically fastened autologous feeder cells for hiPS cell tradition with out extra time wanted for colony formation. Remedy of (2.5% glutaraldehyde or formaldehyde for 10 min) autologous human dermal fibroblasts allowed hiPS cells to stick and develop as undifferentiated colonies characterised by the expression of pluripotency markers reminiscent of alkaline phosphatase, Oct-3/4, and stage-specific embryonic antigen-4.

Transferrin protein (Holo)

30R-AT020 10 gram
EUR 3265
Description: Purified native Human Transferrin protein (Holo)

Bovine Holo Transferrin

7-05368 500mg Ask for price

Bovine Holo Transferrin

7-05369 1gram Ask for price

Bovine Holo Transferrin

7-05370 5grams Ask for price

Holo Transferrin Peptide

abx260005-1gr 1 gr
EUR 648
  • Shipped within 5-10 working days.

Holo Transferrin Peptide

abx260005-500mg 500 mg
EUR 467
  • Shipped within 5-10 working days.

Holo Transferrin Peptide

abx260005-5grs 5 grs
EUR 2082
  • Shipped within 5-10 working days.

Transferrin (HOLO), Human Plasma

7542-100
EUR 164

Transferrin (HOLO), Human Plasma

7542-500
EUR 321

HTF Holo Transferrin Human protein

PROTP02787 Regular: 1g
EUR 577
Description: Human Holo Transferrin is a glycoprotein of approximately 77 kDa containing 679 amino acids.

Cow Holo Transferrin Protein

abx260048-1gr 1 gr
EUR 773
  • Shipped within 5-10 working days.

Cow Holo Transferrin Protein

abx260048-500mg 500 mg
EUR 523
  • Shipped within 5-10 working days.

Cow Holo Transferrin Protein

abx260048-5grs 5 grs
EUR 3084
  • Shipped within 5-10 working days.

Bovine Transferrin, holo (>98%)

TF21-N-10 10 mg
EUR 164

Transferrin, Holo, Human Plasma, purified >97%

TF25-N-BLK blk Ask for price

HTF Holo Transferrin Bovine protein

PROTQ29443 Regular: 1g
EUR 682
Description: Bovine Holo Transferrin is a glycoprotein of approximately 80 kDa.

Purified Human Holo-Transferrin Protein WB +ve Control

TF11-C 100 ul
EUR 286

Recombinant Human Lactoferrin Holo

7-05518 10mg Ask for price

Recombinant Human Lactoferrin Holo

7-05519 100mg Ask for price

Recombinant Human Lactoferrin Holo

7-05520 1gram Ask for price

Lactoferrin protein (Holo)

30-1147 1 g
EUR 329
Description: Purified recombinant Human Lactoferrin protein

Lactoferrin Holo Protein

20-abx262996
  • EUR 328.00
  • EUR 230.00
  • 100 mg
  • 10 mg
  • Shipped within 5-10 working days.

Lactoferrin Holo Protein

abx262996-1gr 1 gr
EUR 885
  • Shipped within 5-10 working days.

Holo TC ELISA KIT|Human

EF010957 96 Tests
EUR 689

Transferrin human

abx082284-10mg 10 mg
EUR 203
  • Shipped within 5-10 working days.

Anti-Ku-holo antibody

STJ16101072 100 µg
EUR 354

Human Holo TC(Holotranscobalamin) ELISA Kit

EH4163 96T
EUR 524.1
  • Detection range: 0.156-10 ng/ml
Description: Method of detection: Coated with Antigen, Competitive ELISA;Reacts with: Homo sapiens ;Sensitivity: 0.094 ng/ml

LTF Lactoferrin Holo Human Recombinant Protein

PROTP02788 Regular: 100mg
EUR 317
Description: Recombinant Human Holo Lactoferrin produced in Plant is a glycosylated mature polypeptide sequence without signal 1-19 peptide, chain 20-712 AA. Having an approximate molecular mass of 77-80 kDa.;The Human Holo Lactotransferrin is purified by proprietary chromatographic techniques.

Human Holo-tanscobalmine(HTC) ELISA Kit

QY-E05534 96T
EUR 361

Transferrin

PR27219 100 mg
EUR 246

Ku-Holo (p70;83)(KU729) Antibody

BNUB0729-100 100uL
EUR 209
Description: Primary antibody against Ku-Holo (p70;83)(KU729), Concentration: 0.2mg/mL

Ku-Holo (p70;83)(KU729) Antibody

BNUB0729-500 500uL
EUR 458
Description: Primary antibody against Ku-Holo (p70;83)(KU729), Concentration: 0.2mg/mL

Ku-Holo (p70;83)(KU729) Antibody

BNUM0729-50 50uL
EUR 395
Description: Primary antibody against Ku-Holo (p70;83)(KU729), 1mg/mL

Ku-Holo (p70;83)(KU729) Antibody

BNC040729-100 100uL
EUR 199
Description: Primary antibody against Ku-Holo (p70;83)(KU729), CF405S conjugate, Concentration: 0.1mg/mL

Ku-Holo (p70;83)(KU729) Antibody

BNC040729-500 500uL
EUR 544
Description: Primary antibody against Ku-Holo (p70;83)(KU729), CF405S conjugate, Concentration: 0.1mg/mL

Ku-Holo (p70;83)(KU729) Antibody

BNC550729-100 100uL
EUR 199
Description: Primary antibody against Ku-Holo (p70;83)(KU729), CF555 conjugate, Concentration: 0.1mg/mL

Ku-Holo (p70;83)(KU729) Antibody

BNC550729-500 500uL
EUR 544
Description: Primary antibody against Ku-Holo (p70;83)(KU729), CF555 conjugate, Concentration: 0.1mg/mL

Ku-Holo (p70;83)(KU729) Antibody

BNC610729-100 100uL
EUR 199
Description: Primary antibody against Ku-Holo (p70;83)(KU729), CF660R conjugate, Concentration: 0.1mg/mL

Ku-Holo (p70;83)(KU729) Antibody

BNC610729-500 500uL
EUR 544
Description: Primary antibody against Ku-Holo (p70;83)(KU729), CF660R conjugate, Concentration: 0.1mg/mL

Ku-Holo (p70;83)(KU729) Antibody

BNC470729-100 100uL
EUR 199
Description: Primary antibody against Ku-Holo (p70;83)(KU729), CF647 conjugate, Concentration: 0.1mg/mL

Ku-Holo (p70;83)(KU729) Antibody

BNC470729-500 500uL
EUR 544
Description: Primary antibody against Ku-Holo (p70;83)(KU729), CF647 conjugate, Concentration: 0.1mg/mL

Ku-Holo (p70;83)(KU729) Antibody

BNC050729-100 100uL
EUR 199
Description: Primary antibody against Ku-Holo (p70;83)(KU729), CF405M conjugate, Concentration: 0.1mg/mL

Ku-Holo (p70;83)(KU729) Antibody

BNC050729-500 500uL
EUR 544
Description: Primary antibody against Ku-Holo (p70;83)(KU729), CF405M conjugate, Concentration: 0.1mg/mL

Ku-Holo (p70;83)(KU729) Antibody

BNC400729-100 100uL
EUR 199
Description: Primary antibody against Ku-Holo (p70;83)(KU729), CF640R conjugate, Concentration: 0.1mg/mL

Ku-Holo (p70;83)(KU729) Antibody

BNC400729-500 500uL
EUR 544
Description: Primary antibody against Ku-Holo (p70;83)(KU729), CF640R conjugate, Concentration: 0.1mg/mL

Ku-Holo (p70;83)(KU729) Antibody

BNC430729-100 100uL
EUR 199
Description: Primary antibody against Ku-Holo (p70;83)(KU729), CF543 conjugate, Concentration: 0.1mg/mL

Ku-Holo (p70;83)(KU729) Antibody

BNC430729-500 500uL
EUR 544
Description: Primary antibody against Ku-Holo (p70;83)(KU729), CF543 conjugate, Concentration: 0.1mg/mL

Ku-Holo (p70;83)(KU729) Antibody

BNC800729-100 100uL
EUR 199
Description: Primary antibody against Ku-Holo (p70;83)(KU729), CF680 conjugate, Concentration: 0.1mg/mL

Ku-Holo (p70;83)(KU729) Antibody

BNC800729-500 500uL
EUR 544
Description: Primary antibody against Ku-Holo (p70;83)(KU729), CF680 conjugate, Concentration: 0.1mg/mL

Ku-Holo (p70;83)(KU729) Antibody

BNCP0729-250 250uL
EUR 383
Description: Primary antibody against Ku-Holo (p70;83)(KU729), PerCP conjugate, Concentration: 0.1mg/mL

Ku-Holo (p70;83)(KU729) Antibody

BNCR0729-250 250uL
EUR 383
Description: Primary antibody against Ku-Holo (p70;83)(KU729), RPE conjugate, Concentration: 0.1mg/mL

Ku-Holo (p70;83)(KU729) Antibody

BNCA0729-250 250uL
EUR 383
Description: Primary antibody against Ku-Holo (p70;83)(KU729), APC conjugate, Concentration: 0.1mg/mL

Ku-Holo (p70;83)(KU729) Antibody

BNCAP0729-100 100uL
EUR 199
Description: Primary antibody against Ku-Holo (p70;83)(KU729), Alkaline Phosphatase conjugate, Concentration: 0.1mg/mL

Ku-Holo (p70;83)(KU729) Antibody

BNCAP0729-500 500uL
EUR 544
Description: Primary antibody against Ku-Holo (p70;83)(KU729), Alkaline Phosphatase conjugate, Concentration: 0.1mg/mL

Ku-Holo (p70;83)(KU729) Antibody

BNCB0729-100 100uL
EUR 199
Description: Primary antibody against Ku-Holo (p70;83)(KU729), Biotin conjugate, Concentration: 0.1mg/mL

Ku-Holo (p70;83)(KU729) Antibody

BNCB0729-500 500uL
EUR 544
Description: Primary antibody against Ku-Holo (p70;83)(KU729), Biotin conjugate, Concentration: 0.1mg/mL

Ku-Holo (p70;83)(KU729) Antibody

BNCH0729-100 100uL
EUR 199
Description: Primary antibody against Ku-Holo (p70;83)(KU729), Horseradish Peroxidase conjugate, Concentration: 0.1mg/mL

Ku-Holo (p70;83)(KU729) Antibody

BNCH0729-500 500uL
EUR 544
Description: Primary antibody against Ku-Holo (p70;83)(KU729), Horseradish Peroxidase conjugate, Concentration: 0.1mg/mL

Ku-Holo (p70;83)(KU729) Antibody

BNC940729-100 100uL
EUR 199
Description: Primary antibody against Ku-Holo (p70;83)(KU729), CF594 conjugate, Concentration: 0.1mg/mL

Ku-Holo (p70;83)(KU729) Antibody

BNC940729-500 500uL
EUR 544
Description: Primary antibody against Ku-Holo (p70;83)(KU729), CF594 conjugate, Concentration: 0.1mg/mL

Ku-Holo (p70;83)(KU729) Antibody

BNC680729-100 100uL
EUR 199
Description: Primary antibody against Ku-Holo (p70;83)(KU729), CF568 conjugate, Concentration: 0.1mg/mL

Ku-Holo (p70;83)(KU729) Antibody

BNC680729-500 500uL
EUR 544
Description: Primary antibody against Ku-Holo (p70;83)(KU729), CF568 conjugate, Concentration: 0.1mg/mL

Ku-Holo (p70;83)(KU729) Antibody

BNC700729-100 100uL
EUR 199
Description: Primary antibody against Ku-Holo (p70;83)(KU729), CF770 conjugate, Concentration: 0.1mg/mL

Ku-Holo (p70;83)(KU729) Antibody

BNC700729-500 500uL
EUR 544
Description: Primary antibody against Ku-Holo (p70;83)(KU729), CF770 conjugate, Concentration: 0.1mg/mL

Ku-Holo (p70;83)(KU729) Antibody

BNC880729-100 100uL
EUR 199
Description: Primary antibody against Ku-Holo (p70;83)(KU729), CF488A conjugate, Concentration: 0.1mg/mL

Ku-Holo (p70;83)(KU729) Antibody

BNC880729-500 500uL
EUR 544
Description: Primary antibody against Ku-Holo (p70;83)(KU729), CF488A conjugate, Concentration: 0.1mg/mL

Ku-Holo (p70;83)(KU729) Antibody

BNC810729-100 100uL
EUR 199
Description: Primary antibody against Ku-Holo (p70;83)(KU729), CF680R conjugate, Concentration: 0.1mg/mL

Ku-Holo (p70;83)(KU729) Antibody

BNC810729-500 500uL
EUR 544
Description: Primary antibody against Ku-Holo (p70;83)(KU729), CF680R conjugate, Concentration: 0.1mg/mL

Recombinant Human Transferrin

7-06703 100mg Ask for price

Recombinant Human Transferrin

7-06704 1gr Ask for price

Recombinant Human Transferrin

7-06705 10gr Ask for price

Human Apo Transferrin

7-04408 500mg Ask for price

Human Apo Transferrin

7-04409 1gram Ask for price

Human Apo Transferrin

7-04410 5grams Ask for price

Human Transferrin Antibody

11641-05011 150 ug
EUR 217

Human Transferrin Antigen

IM119 1 ml
EUR 285
  • Product category: Quality control/standard for molecular biology applications
Description: Human Transferrin Antigen by Cygnus Technologies is available in Europe via Gentaur.

Transferrin, Human Plasma

TF16-N 10 mg
EUR 164

Recombinant Human LTF Holo Protein, Untagged, Rice-100mg

QP12596-100mg 100mg
EUR 201

Recombinant Human LTF Holo Protein, Untagged, Rice-10mg

QP12596-10mg 10mg
EUR 155

Recombinant Human LTF Holo Protein, Untagged, Rice-1gram

QP12596-1gram 1gram
EUR 663

Mouse Anti Hcg Holo Monoclonal Antibody

CABT-49228MH 0.5 mg
EUR 715

Human Transferrin, CF488A conjugate

00081 1mg
EUR 149
Description: Minimum order quantity: 1 unit of 1mg

Human Transferrin, CF543 conjugate

00082 1mg
EUR 149
Description: Minimum order quantity: 1 unit of 1mg

Human Transferrin, CF568 conjugate

00083 1mg
EUR 149
Description: Minimum order quantity: 1 unit of 1mg

Human Transferrin, CF594 conjugate

00084 1mg
EUR 149
Description: Minimum order quantity: 1 unit of 1mg

Human Transferrin, CF640R conjugate

00085 1mg
EUR 149
Description: Minimum order quantity: 1 unit of 1mg

Human Transferrin, CF680R conjugate

00086 1mg
EUR 149
Description: Minimum order quantity: 1 unit of 1mg

Human Transferrin, CF750 conjugate

00087 1mg
EUR 155
Description: Minimum order quantity: 1 unit of 1mg

Human Transferrin ELISA Kit

E-80TX 1 x 96 well plate
EUR 370

Human Transferrin ELISA kit

E01T0130-192T 192 tests
EUR 1270
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A sandwich ELISA for quantitative measurement of Human Transferrin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Transferrin ELISA kit

E01T0130-48 1 plate of 48 wells
EUR 520
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A sandwich ELISA for quantitative measurement of Human Transferrin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Transferrin ELISA kit

E01T0130-96 1 plate of 96 wells
EUR 685
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A sandwich ELISA for quantitative measurement of Human Transferrin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Transferrin (TF) Protein

20-abx167810
  • EUR 495.00
  • EUR 244.00
  • EUR 1414.00
  • EUR 578.00
  • EUR 356.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug
  • Shipped within 5-15 working days.

Human Transferrin (TF) Protein

20-abx069402
  • EUR 411.00
  • EUR 217.00
  • EUR 1052.00
  • EUR 467.00
  • EUR 300.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug
  • Shipped within 5-7 working days.

Human Plasma Transferrin Protein

abx060950-100mg 100 mg
EUR 370
  • Shipped within 5-10 working days.

Human Transferrin (TF) Protein

abx670229-100mg 100 mg
EUR 523
  • Shipped within 1 week.
Moreover, hiPS cells cultured on chemically fastened feeders shaped teratomas in vivo, characterised by all three germ layers. The chemically fastened autologous feeders could also be used as an alternative choice to giant scale tradition of hiPS cells as a handy in-house and a cheap technique.

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