Purified Native dsDNA, Calf Thymus, ELISA/Antigen Grade

Properties

Writes: Type i

Quality level: 300,

Form: fibers

Colour: White

Storage temperature: 2-8 ° C

Related categories: Nucleosides and nucleotides

Description

Application

Calf Thymus DNA (CT-DNA) is a natural DNA widely used in studies of anticancer agents that bind DNA and agents that bind DNA that modulate the structure and function of DNA. Calf thymus DNA is also used in physicochemical studies of the behaviour of DNA in solution.

Packaging

  • 1.5g in a polyethene bottle
  • 50, 100, 500 mg in a polyethene bottle

Quality

“Highly polymerized”

Physical properties

  • λmax 259 nm (100 mM phosphate buffer, pH 7.0).
  • Calf thymus DNA is 41.9 mole% G-C and 58.1 mole% A-T.1 An OD of 1.0 at 260 nm corresponds to ~ 50 µg of double-stranded DNA.

Physical form

Fibrous preparation

Preparation note

  • This product is prepared from calf thymus tissue of unspecified sex.
  • This product is extracted by a method that causes shearing producing a highly polymerized mixture of single and double-stranded DNA. However, double-stranded DNA is the predominant form.

Reconstitution

DNA solutions have been successfully stored for several months at 4 C, pH 7.5-8, in 10 mM Tris with 1 mM EDTA and no bacteriostatic agent. At low concentrations (μg / ml), DNA tends to absorb onto the surfaces of plastic tubes. Storing DNA in highly alkaline solutions is not recommended as DNA tends to degrade at an alkaline pH greater than 8.0.

This product can be dissolved at 2 mg/ml in water. To further reduce DNA shear, do not use ultrasound or shaking. Gentle inversion overnight at 0-4 ° C is recommended to fully solubilize the DNA. The presence of 1 mM EDTA is recommended to prevent nucleases from degrading DNA.

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